The 2016 Inc. 5000 has been released, with Franklin-based natural health company Axe Wellness the top Middle Tennessee company in the ranking.

Issued annually, the Inc. 5000 ranks the fastest growing private companies in the United States.

Altogether, there are 53 Nashville-area private companies on the 2016 Inc. 5000.

Axe Wellness, whose website has 6 million monthly visitors, ranks No. 130 on the list after experiencing three-year growth of 2,645 percent and revenue of $11.6 million.

The company was founded in 2010 by Josh Axe, who is a doctor of natural medicine and chiropractic and a clinical nutritionist.

Commodore Leasing, a Franklin-based franchisee of Aaron’s and LifeLine Smartphone & Tablet Repair, is the second-highest ranking local business. Commodore ranks No. 193, with three-year growth of 1,933 percent and revenue of $3.6 million. The company was founded in 2011.

A. Marshall Family Food, the Franklin-based operator of Puckett’s Boat House and Puckett’s Grocery and Restaurant, ranks No. 653 on the Inc. 5000. The company reported three-year growth of 598 percent and revenue of $18.8 million. Founded in 2010, the company also operates Scout’s Pub and Homestead Manor.

Jones Therapy Services, a Franklin-based provider of physical therapy, ranks No. 672. Jones reported three-year growth of 583 percent and revenue of $2.2 million. The company was founded in 2009.

Aerial Development Group, a Nashville-based urban infill homebuilding company, ranks No. 769. Led by Britnie Turner, Aerial had three-year growth of 517 percent and revenue of $27 million. The company was founded in 2009.

The local entity with the largest revenues on this year’s list is Vaco, a Brentwood-based business products company. Vaco ranks No. 4,205 on the Inc. list. It reported three-year growth of 65 percent and revenue of $291.3 million. The company was founded in 2002.

Nashville-based StyleBluePrint has the smallest revenues of area companies on the list, ranking No. 1,933. It reported three-year growth of 192 percent and revenue of $2 million.

Of note, the Nashville chapter of Entrepreneur Organization landed 17 members on the list.

“As the third largest EO chapter in the world, it’s not a surprise to see so many of our members’ companies represented on the Inc. 500|5000 list, but it’s always a thrill,” said John Kepley, EO Nashville president and CEO of Teknetex. “This is a big win for Nashville, too, and it solidifies Music City’s prominence in the entrepreneurial world.”